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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (3) Autor Neves,A.L.A. (3) Pereira,L.G.R. (3) Santos,R.D. (3) Aragão,A.S.L. (2) Araújo,G.G.L. (2) Azevêdo,J.A.G. (2) Brandão,L.G.N. (2) Brandão,W.N. (2) Aragão,A.L.S. (1) Chizzotti,M.L. (1) Ferreira,A.L. (1) Oliveira,G.F. (1) Souza,R.A. (1) Voltolini,T.V. (1) Mais... Palavra-chave Ganho de peso (2) Ovino (2) Agave sisalana (1) Alimento alternativo (1) Benefício líquido (1) Composição bromatológica (1) Consumo (1) Nutrientes (1) Resíduo agroindustrial (1) Semiárido (1) Sisal (1) Subproduto (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2013 (1) 2011 (2) País Brazil (3) Idioma Português (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 66 Primeira ... 1 2 3 4 ... Última Structuration génétique d'une collection de souches de Vibrio. Contribution à la mise au point d'outils de typage moléculaire pour l'aide au diagnostic ArchiMer Jaffres, Emmanuel. Les bactéries du genre Vibrio représentent le groupe le plus fréquemment associé à des maladies infectieuses chez les mollusques marins. Ainsi chez l'huître creuse Crassostrea gigas, la majorité des Vibrios isolés d'animaux sains ou malades sont phénotypiquement classés dans un groupe de souches apparentées à Vibrio splendidus. Les récentes études phylogénétiques pars séquençage du gène gyrB, ont permis de distinguer plusieurs espèces au sein de ce groupe (V. splendidus, V. chagasii, V. cyclitrophicus, V. crassostreae, V. gigantis). Toutefois, il subsiste toujours des imprécisions dans l'arbre phylogénétique gyrB. En effet, un groupe polyphylétique de 4 espèces reste jusqu'à présent on distinguables (V. tasmaniensis, V. kanaloae, V. pomeroyi, V. lentus).... Tipo: Text Palavras-chave: Mortalités estivales; Huître creuse; Crassostrea gigas; Vibrio spendidus; PCR-RFLP; Séquençage; Gène ADNr-16S; GyrB; Macroarrays. Ano: 2005 URL: http://archimer.ifremer.fr/doc/00034/14479/11779.pdf Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups Genet. Mol. Biol. Hiragi,Cássia de Oliveira; Miranda-Vilela,Ana Luisa; Rocha,Dulce Maria Sucena; ,Silviene Fabiana de; Hatagima,Ana; Klautau-Guimarães,Maria de Nazaré. Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution of these variants. We described the distribution frequency of CAT (21A/T), SOD2 (Ala9Val), GPX1 (Pro198Leu), GSTM1 and GSTT1 polymorphisms in three Brazilian population groups: Kayabi Amerindians (n = 60), Kalunga Afro-descendants (n = 72), and an urban mixed population from Federal District (n = 162). Frequencies of the variants observed in Kalunga... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; PCR-RFLP; Gene polymorphisms; Brazilian ethnicities; Population genetics; Pharmacogenetics. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100003 The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini) Genet. Mol. Biol. Fagundes,Valéria; Nogueira,Cristina Dornelas de Andrade. Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic... Tipo: Info:eu-repo/semantics/other Palavras-chave: Akodon cursor; Akodon montensis; PCR-RFLP; Sigmodontinae. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031 The use of PCR-RFLP as an identification tool for two closely related species of bats of genus Platyrrhinus Genet. Mol. Biol. Ferreira,Juliana Machado; Martins,Felipe de Melo; Ditchfield,Albert; Morgante,João Stenghel. The bat species Platyrrhinus lineatus and P. recifinus (Phyllostomidae: Stenodermatinae) are ecologically important because of their capacity for seed dispersal. P. recifinus is endemic to the Atlantic rain forest and is considered vulnerable by the IUCN. The lack of distinct morphological features makes identification of the two species a difficult task. This study was aimed at testing the hypothesis that these are actually two distinct species by using PCR-RFLP of the mitochondrial cytocrome b gene. The results showed no shared haplotypes, demonstrating that these are, in fact, two distinct species. No polymorphism was obtained for P. recifinus, which could be a sign of low genetic diversity in this threatened species. Tipo: Info:eu-repo/semantics/article Palavras-chave: Platyrrhinus; PCR-RFLP; Identification; Phyllostomidae. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100021 Use of pcr-rflp of thefla a gene for detection and subtyping of Campylobacter jejuni strains Potentially related to Guillain-barré syndrome, isolated from humans and animals BJM Scarcelli,E.; Piatti,R.M.; Harakava,R.; Miyashiro,S.; Campos,F.R.; Souza,M.C.A.; Cardoso,M.V.; Teixeira,S.R.; Genovez,M.E.. The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when... Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; PCR-RFLP; Subtyping; Guillain-Barré Syndrome. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029 β-casein gene polymorphism permits identification of bovine milk mixed with bubaline milk in mozzarella cheese Genet. Mol. Biol. Otaviano,Antonio R.; Lima,André Luis F.; Laureano,Monyka M.M.; Sena,Janete A.D.; Albuquerque,Lúcia G. de; Tonhati,Humberto. Mozzarella cheese is traditionally prepared from bubaline (Bubalus bubalis) milk, but product adulteration occurs mainly by addition of or full substitution by bovine milk. The aim of this study was to show the usefulnes of molecular markers to identify the admixture of bovine milk to bubaline milk during the manufacturing process of mozzarella cheese. Samples of mozzarella cheese were produced by adding seven different concentrations of bovine milk: 0%, 1%, 2%, 5%, 8%, 12% and 100%. DNA extracted from somatic cells found in cheese were submitted to PCR-RFLP analysis of casein genes: α-s1-CN - CSN1S1 that encompasses 954 bp from exon VII to intron IX (Alu I and Hinf I), β-CN - CSN2 including 495 bp of exon VII (Hae III and Hinf I), and κ-CN - CSN3,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Casein; Gene polymorphism; Mozarella; Cheese authentication; PCR-RFLP. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500017 Registros recuperados: 66 Primeira ... 1 2 3 4 ... Última

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